制备用球形填料

Preparative spherical packing materials

制备用球形硅胶填料的尺寸是分别为10μm、15μm、20μm、50μm、75μm、150μm的6个种类。任何粒径的填料都和分析用填料一样,进行了严格的品质管理。粒径为50μm、75μm、150μm的填料,其粒径分布狭窄,是性价比很高的产品。其中50μm的YMC S-50填料,由于拥有适当的粒径和性价比,所以被广泛应用于制备色谱。在此,特别介绍YMC S-50的各种特点。



YMC S-50填料

  • YMC S-50硅胶为粒径50um,微孔径12nm(120Å)的制备用球形填料。
  • 由于粒径分布狭窄,因此采用震动拍打(Tapping)的方式来装填也能获得很高的柱效。
  • 由于是在严格的品质管理下进行的大量生产,因此不但性能卓越,而且经济实惠。
  • 不仅可用于工业制备生产,还可用于半制备纯化。
  • 最适合和YMC耐压玻璃柱一起用于制备纯化。

通过震动拍打装填实现高柱效

Comparison between tapping and slurry

分别使用震动法和匀浆法将YMC S-50填料进行装柱,并对两根柱子的柱效加以比较。可以看出简单的震动填装法也能达和匀浆填装法相同的柱效。

Column : ODS-A(S-50µm , 12nm (120A)), 500X20mmI.D.
Eluent : methanol/water (75/25)
Detection : 10mL/min
Flow rate : UV at 254 nm , 0.32AUFS
Sample : 1. Uracil 2. Methyl benzoate 3. Toluene 4. Naphthalene
Injection : 16µL

和不定形填料相比可获得更高的理论踏板数

Higher theoretical plate number is achieved as compared with irregular packing materials

不定形的40/60μm填料和YMC S-50硅胶填料同时用震动法来分别进行装填,并对柱效进行比较,可以发现具有狭窄粒径分布的球形填料YMC S-50能得到更高的柱效。

Column size : 500 x 20 mmI.D.
Eluent : methanol/water (85/15)
Detection : 40mL/min
Flow rate : UV at 254 nm , 0.32AUFS
Sample : 1. Uracil 2. Methyl benzoate 3. Toluene 4. Naphthalene
Injection : 100µL

和不定形填料相比可获得更高的理论踏板数

Column Size Graph

用YMC S-50硅胶填料分别装填不同尺寸的制备柱,并对其柱效进行比较。为了得到更直观的比较数据,实际上我们根据色谱柱的横截面积比对流动相的流速和样品的上样量进行了调整。可以看到,用YMC S-50装填的各种尺寸的制备柱都能得到很高的柱效。

Column : YMC Pack ODS-AQ (S-50µm , 12nm (120Å) )
Eluent : methanol/water (85/15)
Detection : UV at 254 nm , 0.16AUFS
sample : 1. Methyl benzoate 2. Toluene

球形硅胶填料的物理性能数值

YMC Stationary Phase for Preparative Chromatography

PRODUCT CODE PHASE(silica unless noted) PORE SIZE(Å) PARTICAL SIZE(µm) CARBON LOAD(%) pH
ProC18 AS perfect endcapping to minimize the effect of residual silanols. ProC18 sets new standards for all types of organic molecules 120 10 16 2-8
ODS-A AA one of YMC's international bestsellers, traditional high performance C18 column 120 10,15,20,50,75,150 17 2-7.5
200 10,15,20 12
300 10,15,20 7
ODS-AQ AQ hydrophilic C18 surface, metabolite recognition for aqueous eluent systems, substanitially increased retention of polar compounds 120 10,15,20,50 14 2-7.5
200 10,15 10
Polymer C18 PC a high efficiency C18 on a polymeric support(no silanols) that is totally inert and enhances selectivity for aromatics, pH2-13 - 10 C18=10% 2-13
C8(Octyl) OC C8 fully endcapped, high coverage monomeric bonding chemistry 120 10,15,20 10 2-7.5
200 10,15,20 7
300 10,15,20 4
YMCbasic BA popular product with large customer base in the US, complementary selectivity pattern for basic compounds proprietary 10 7 2-7.5
Phenyl PH monomeric phenyl bonding, endcapped 120 10,15,20 9 2-7.5
C4(Butyl) BU C4 fully endcapped, high coverage monomeric bonding chemistry 120 10,15,20 7 2-7.5
200 10,15,20 5
300 10,15,20 3
C1(TMS) TM trimethyl silane, excellent hydrolytic stability 120 10,15,20 4 2-7.5
Amino NH primary amino derivative, high coverage monomeric bonding chemistry 120 10 3 2-7.5
Cyano CN fully endcapped, useful also for SFC applications 120 10,15,20 7 2-7.5
Diol DN for normal phase applications, good selectivity without excessive retention 120 10,15 - 2-7.5
200 15
YMC Chiral Prep CD ST ST cyclodexstrin based stationary phase for chiral compounds 120 10,20 50 - 2-7
YMC Chiral Prep CD PM PM phenyl modified cyclodextrin based phase with different selectivity compared to ST 120 10,20 50 - 2-7
Silica SL ultra high purity, high mechanical stability 60 10,15,75,150 - 2-7.5
120 10,15,20,50,75,150
200 10,15
300 10,15
1000 10.50
PROTEIN-RP PR This is a silica-based reversed-phase material designed for separation of proteins or peptides. It has strong acid resistance. 200 15 4 1.5-7.5